article – Page 7 – asiatic insights

Globalization and Epidemics

With the ever-expanding globalization and sophisticated transportation networks, the world has become a tighter global village. Human mobility has increased by more than a thousand fold since the 19^th century, and the number of people traveling across the world is increasing 10% annually. In addition, economic globalization has helped to increase shipping traffic by more than 27% in the past two decades.

In response, international health agencies have taken steps to implement evidence-based health policies to prevent epidemic outbreaks that may result from increased international travel. Continental populations are no longer isolated, and therefore diseases that were once confined to their continent of origin can spread quickly across the world. That is, air and water transport systems have reduced such confinement.

For example, the SARS outbreak from a single infected physician from Guangdong spreading to 16 hotel guests in Hong Kong, who in turn spread the disease to other countries such as Vietnam, Canada, and Singapore, led to the spread of the disease to 26 countries, 5 continents, and 8,000 people. This case clearly shows the power of globalization. Another case is the Ebola virus outbreak. The outbreak started from Western Africa, where the disease was rampant at the time. A traveler passing through the affected area got the disease and brought it to the US, making it a pandemic.

These cases show that it is impossible to control travel of people and thus that the spread of diseases from a remote location to major cities must be controlled for. Therefore, agencies such as the World Health Organization and the Centers for Disease Control and Prevention should coordinate technical assistance to limit the spread of diseases at the earliest known outbreak.

S.T. Park

EQUALITY AND EMPLOYMENT

Xiaolin Qi

There is no such thing as a true transcendentalism, and therefore there is no true individual.

Society creates humans, and people tend to rely on society. And regardless of how people view their communities, it is what their lives depend on. With such a system, there will always be issues lingering around it, causing minor and major side effects. Then it becomes people’s job as a component of this system to diagnose problems and try to solve them.

With the economy struggling to recover from the subprime crisis and the failure of the Chinese stock market, job opportunities have become one of the most serious social issues in recent years. In both developed and developing countries, unemployment rates have skyrocketed in the last five years. Even in the U.S., the unemployment rate has shown no significant reduction since the Obama administration. Considering the overall status of the global economy, job opportunities may not recover for up to a decade. Clearly it is a problem when 99% of the population have to worry about their everyday lives.

With a majority of people worried about their jobs, rich people remain in control of the market. From wealth distribution to labor force employment, all issues facing the common people are not at all a concern for these individuals. The gap in employment rates between America’s highest- and lowest-income families has stretched to its widest levels since officials began tracking it a decade ago. According to government data reported by the Associated Press, unemployment rates for lowest-income families (those earning less than $20,000) have reached 21%, nearly matching the rate for all workers during the Great Depression. The unemployment rate for the poor has skyrocketed since the 2008 financial crisis and has never fully recovered, even under the Obama administration.

The unemployment rate is also a huge problem when it comes to race. Data from the Bureau of Labor Statistics cover the age range from 16 to 19 and indicate that, for whites in this age range, the official unemployment rates are about 15.7% for Hispanics and 20.8% for African-Americans. This huge gap has been around for many decades and still has considerable influence on the economy. For every level of educational attainment, blacks have unemployment rates that are similar to or higher than less educated white counterparts. For instance, whites with only high school diplomas have unemployment rates similar to blacks with college degrees: 4.6% versus 4.1%. The unemployment rate has been a problem for minority groups in America, and it is expected to remain a serious problem in the foreseeable future.

There is no doubt that the economy has been expanding in recent years. However, there remain serious problems for people without jobs. It is possible that this economic expansion may reduce the unemployment rate, but this will ultimately depend on the speed at which the employment-to-population ratio increases and the increase in the productivity of the workers added as the labor market expands. Based on the current economic situation, the market is not looking good for people.

The Urban Society

This is the place; this is the age, Oh my ancient sage!

Where man lives hiding in his hole, deep inside this concrete jungle,

He strains his head to see the sunset, for he cannot see beyond this golden cage,

The sunrise he can never see, unnatural lights flicker and disrupt his cycle every night.

This is the place; this is the age, Oh my holy father!

Where everyone is in a hurry, the stress runs high,

Where money is the driving force, for it, one kills another,

Where technological intelligence thrives, and spiritual maturity is about to die.

This is the place; this is the age, Oh my king!

Where my ruler is a criminal, but I’m not bothered, I am mentally sedated,

The moral implications of my actions don’t bother me now,

I am here and I live for now, there is no God I fear, everyday here, truth is adulterated.

This is the place; this is the age, Oh my love!

Where I can break every marriage vow, showing you the glitter of diamonds,

Where I can use words like ‘divorce’ loosely for our marriage was a contract honey,

not made high above.

This is the place; this is the age, Oh mother earth!

Where conceited men like I, abuse you and pollute you for our greed,

We spit on the same soil we sow, it is from here that men like us grow,

We will be the cause of your death, but you will continue to give us birth.

This is the place; this is the age, Oh my guru!

Where my teacher despises fools, but hates that I am clever,

Where my thirst for knowledge, is my arrogance,

Where sticking to my guns, is my belligerence.

When the world around me is so unreal, where do I start?

Our complex minds have gotten us here; let’s give a chance to our heart,

Let emotions be the voice of reason, let your instincts be the guiding ray,

Through those clouds of uncertainty, simplicity is the only way,

Too much oo much gyaan for you to handle, now go, have a nice day!

Student: Ameya Malkan

Invisible Chains: The Global Crisis of Human Trafficking and Modern Slavery

Brian Tran

Today, over 40 million people around the world are victims of modern slavery. This is more than at any other time in human history. Despite being illegal in almost every country, human trafficking and modern slavery continue to expand, often hidden in plain sight. These crimes involve the act of people exploitation through fraud, force or coercion, whether for forced labor, sexual exploitation, or domestic servitude. This issue impacts on nearly every country in the world and targets the most vulnerable groups such as women, children, and migrants.

Human trafficking is the illegal trade of people, often involving transportation across borders, but it can also take place within the entire country. Victims are often offered with lucrative benefits like promised jobs, education, or simply a better life, only to be exploited once they are under the control of traffickers. Modern slavery is a broader term that includes trafficking but also covers forced marriage, child labor, and other forms of exploitation that involve control and abuse.

There are several roots that contributes to this global spread issue. One of the most significant ones is poverty. When people lack opportunities, education, or protection, they are more vulnerable to be lured into trafficking schemes. Gender inequality is also a key factor in which women and girls make about 71% of all trafficking cases worldwide. They often be exploited as sexual tools. Armed conflict, political instability, and refugee crises have also increased trafficking, as displaced people become easy targets. Meanwhile, in many regions with corrupt officials and weak legal system, this act is allowed to act with impunity.

Modern slavery is also associated with many forms. Forced labor is one of the most common, where people are made to work in factories, farms, or construction sites, often under inhuman conditions with such a little pay or even zero pay. Sex trafficking is also another widespread form which involves forcing individuals (often women and girls) into prostitution, pornography, or exploitation of sex work. Children are also victims. They are trafficked for labor, begging, or even forced into conflict as child soldiers. All these crimes are just to earn money based on blood and the suffering of others. In some cases, domestic workers are trafficked into homes where they are abused and taken over basic human rights.

In response to these staggering crimes, many governments and organizations have begun to respond to this crisis. The United Nations has included the goal to end modern slavery by 2030 in its Sustainable Development Goals. Several countries like UK, France, Germany, or Benin have passed stronger anti trafficking laws and established rescue and rehabilitation programs to support survivors. However, progress remains slow. Fewer than 1% of victims are ever rescued, and traffickers often operate across borders, making prosecution more difficult.

So, what could we do to reduce this problem? We must raise the public awareness about this issue. It could be education campaigns in schools, communities, and online to help people understand the signs of trafficking and how to prevent it; or law enforcement must be trained and resourced to go after traffickers and protect the survivors. In addition, we must also demand more ethical business practices in which companies must ensure their supply chains are free of forced labor. Finally, empowering at risk populations with education, job opportunities, legal protection is key to break the cycle.

Human trafficking is a modern form of slavery – more cruel, illegal and often invisible. But it is not unstoppable. By working together as individuals, communities, and nations, we can fight back against this injustice and give freedom and dignity back to millions who deserve to have it but have been denied both for far too long.

Feasibility Testing of a Glass Microsphere-Based Lactase Immobilization System for Domestic Use

Feasibility Testing of a Glass Microsphere-Based Lactase Immobilization System for Domestic Use

Guochen Dong, Yuanzhao Pan

Introduction

Lactose intolerance is a syndrome that describes people who have several symptoms when they uptake products that have lactose, a kind of disaccharide that can be broken into one molecule of glucose and one molecule of galactose. This is normally done by a kind of enzyme called lactase. Many people, however, have defficient lactase, which means that they can’t normally break down lactose when they intake diary products, especially milk.(Malik and Panuganti, 2023) Having milk can lead to a wide range of symptoms including some common ones like nausea and diarrhea(Chevalier told me). This may pose a big threat to human nutrition because milk plays a crucial part in a healthy diet by providing proteins, and Lactose introlerance is prevalent in Asia.(Vandenplas 2015) According to worldpopulationreview.com, in 2024 there are about 85% of Chinese people who suffer from lactose intolerance.

Recently there are already commercial applications of using lactase for lactose intolerant people, such as directly producing lactose-free milk or providing lactase drops for civil use. In industrial production of lactose-free milk, both mobilized and immobolized lactase are commonly used. Immobilized lactase in lactose-free milk production, specifically, along with the whole field of immobilized enzymes, has recently gained significant focus, not only because of its application on industrial manufacture, but also has potential in bioengineering and other fields.(Shukla and Wierzbicki, 1975)

In immobilizing lactase, there are many possible supporting materials, such as porous glass, alginate, and cellulose. (Yılmaz-Karaoğlu et al., 2022) There are many researches that have focused on determining the effects of types of materials to the activity of lactase. According to researches, lactase immobilized in glass microspheres has high enzyme activity, and can be effective after dozens of rounds. (Ko et al., 2018)

Aim

The experiment aims to design a home-based device to remove lactose from commercially sold milk products. We decided to make a product that includes a container and immobilized lactase for civil use. In our first ideas, the product can be used by pouring milk inside and wait for the lactose to be hydrolyzed. Its effectiveness may also persists after tens of such procedures. Because of the high enzyme activity on glass supports, we decided to use glass microspheres as our lactase supporting platform for our product.

Product and Experiment design

Based on the description in the essay, we will need to conform to the following procedures to immobilize lactase.

Preparation of Glass Microspheres:

20 cm³ (30 g) of glass microspheres were cleaned with concentrated nitric acid for 48 hours.

After several washes in double-distilled water, the microspheres were immersed for 3 hours at 60°C in a 10% (v/v) 3-aminopropyltriethoxysilane (APTS) aqueous solution (15 mL) to modify the surface for enzyme immobilization. The pH of the silane solution was adjusted to pH 4 using 6 N HCl.

The glass microspheres were then washed with double-distilled water and dried overnight in an oven at 80°C.

Activation of Carriers:

Each carrier, including the prepared glass microspheres, was treated with 20 mL of 2.5% glutaraldehyde aqueous solution for 60 minutes at room temperature.

Washing:

The carriers were washed with double-distilled water to remove any residual glutaraldehyde.

Incubation with Lactase:

The activated carriers were incubated for 16 hours at 4°C with 1 mg of lactase dissolved in 10 mL of 0.1 M phosphate buffer (pH 6.0, adjusted with sodium hydroxide and sodium carbonate).

Removal of Unbound Lactase:

Any unbound lactase was removed by washing the carriers with 0.1 M phosphate buffer.

Storage:

The lactase-linked carriers were stored at 4°C until use. (Ko, Chih-Yuan, et al, 2018)

Next, we will integrate a heating system and an agitator to form the prototype of our final product. For our experiment, we will only need a constant heating platform and a stirring stick to simulate the process.

First, we will need to test whether the immobilize lactase is feasible to digest lactose. Since it only required us to determine whether lactose is digested and do not require us to do quantitative analysis, considering the tight budget as a high school student, we decided to use Benedict’s test, which is much cheaper than assay kits, to determine the feasibility of the approach. In order to exclude the interference of lactose, we have done a small pre-experiment. We have configured 4.5% lactose solution, 9% glucose solution (due to the lack of galactose, we just use glucose to substitute galactose, since they are both reducing sugar), 2.25% lactose and 4.5% glucose solution (simulate half of the lactose is digested), and pure water. Through Benedict’s test, the result shows that there is an observable difference between each solution. This is because, when lactose is digested by lactase, it will hydrolysis into glucose and lactose, so the concentration of sugar will be the twice of its original. Based on the result, we decided to use Benedict’s test for our initial proof of feasibility.

Second, we will conduct experiments to find the optimal temperature for the process. Using the constant heating plate, we will set several experimental groups with same amount of milk, lactase, and reaction time, but different temperatures. The control groups will be at the room temperature, and data will be recorded. The final evaluation of lactose residual will measure by the Glucose GOD/POD assay kit. The glucose GOD-POD assay utilizes glucose oxidase (GOD) to catalyze the oxidation of glucose to gluconolactone and hydrogen peroxide, which, in the presence of peroxidase (POD), reacts with a chromogenic substrate to produce a colorimetric change proportional to the glucose concentration. This method is highly specific to glucose due to the enzyme’s specificity, allowing for accurate quantification even in complex matrices like milk. The sensitivity of the GOD-POD assay enables the detection of low glucose concentrations, making it suitable for analyzing lactose-hydrolyzed milk products where glucose levels are indicative of the extent of lactose hydrolysis. The accuracy of this method has been validated against standard analytical techniques, such as high-performance liquid chromatography (HPLC), demonstrating its reliability for quantitative glucose analysis in dairy products. (Ambade Vivek, et al, 1998) The difference of concentration of glucose can represent the extent of lactose digestion, since only the breakdown of lactose can increase the concentration of glucose.

Third, after finding out the approximate optimal temperature. We will conduct another experiment which will use focus on testing the reaction time for the prototype. Obtaining sample of different time point through one reaction, we will use same assay to derive data.

In the final stage, we will focus on analyzing the data collected from our experimental trials to determine the optimal conditions for lactose digestion. It will be carried out using graphing software. The graph and data will reveal us the final optimal operation condition for the process and the efficiency of our products, with the ultimate goal of establishing the most effective parameters for lactose digestion in our home-based lactose removal device.

Given our status as high school students with limited budgets, our approach to this research project is designed to be as cost-effective as possible. We intend to make full use of the laboratory resources available at our school, thereby minimizing external expenses. Additionally, we will create a detailed budget plan to account for necessary materials such as milk samples. In sourcing these materials, we will seek out affordable options that do not compromise the integrity of our experiments. This careful financial planning and resource management will ensure that our project remains viable, enabling us to develop a practical solution for lactose intolerance that can be implemented within the confines of a home kitchen. This focus on creating a home edition lactase exclusion machine underscores our commitment to making lactose digestion technology accessible and affordable for everyday use.

Innovation and Impact

We have searched about lactases for civil use and found that they are mostly in dropping liquid form. This can be widely applicable and quite effective, but using immobilized lactase device can have better performance in effectivity, reusability, larger working range and possibly lower prices. Our design may provide an exploration of innovation in such civil used devices. If it can be proven of relatively high efficiency, low negative effects to milk, and relatively low prices, it may have possible commercial use in daily application, especially may serve as a public device in schools for students who are lactose intolerance.

Reference List

AMBADE, VIVEK N, et al. “METHODS for ESTIMATION of BLOOD GLUCOSE : A COMPARATIVE EVALUATION.” Medical Journal Armed Forces India, vol. 54, no. 2, Apr. 1998, pp. 131–133, https://doi.org/10.1016/s0377-1237(17)30502-6.

Horner, T. W., et al. “β-Galactosidase Activity of Commercial Lactase Samples in Raw and Pasteurized Milk at Refrigerated Temperatures.” Journal of Dairy Science, vol. 94, no. 7, 1 July 2011, pp. 3242–3249, www.sciencedirect.com/science/article/pii/S002203021100316X#bib0090, https://doi.org/10.3168/jds.2010-3742.

Ko, Chih-Yuan, et al. “Lactose-Free Milk Preparation by Immobilized Lactase in Glass Microsphere Bed Reactor.” Food Biophysics, vol. 13, no. 4, 11 July 2018, pp. 353–361, https://doi.org/10.1007/s11483-018-9541-8. Accessed 5 June 2022.

Shukla, Triveni Piasad, and Leopold E. Wierzbicki. “Beta‐Galactosidase Technology: A Solution to the Lactose Problem.” C R c Critical Reviews in Food Technology, vol. 5, no. 3, Jan. 1975, pp. 325–356, https://doi.org/10.1080/10408397509527178.

Vandenplas Y. (2015). Lactose intolerance. Asia Pacific journal of clinical nutrition, 24 Suppl 1, S9–S13. https://doi.org/10.6133/apjcn.2015.24.s1.02

Birch, G G, et al. Enzymes and Food Processing. London: Applied Science, 1981.

World Population Review. “Lactose Intolerance by Country 2020.” Worldpopulationreview.com, 2021, worldpopulationreview.com/country-rankings/lactose-intolerance-by-country.

Malik, T. F., & Panuganti, K. K. (2023). Lactose Intolerance. In StatPearls. StatPearls Publishing.

Yılmaz-Karaoğlu, Sümeyye, et al. “Lactose Hydrolyzing Activity of the Lactase Immobilized Polycaprolactone and Silk Fibroin-Based Nanofiber and Nitrocellulose Membrane.” Food Bioscience, vol. 49, Oct. 2022, p. 101828, https://doi.org/10.1016/j.fbio.2022.101828. Accessed 30 Jan. 2023.